Associations of ambient particulate matter with homocysteine metabolism markers and effect modification by B vitamins and MTHFR C677T gene polymorphism.

Evidence concerning effects of ambient air pollution on homocysteine (HCY) metabolism is scarce. We aimed to explore the associations between ambient particulate matter (PM) exposure and the HCY metabolism markers and to evaluate effect modifications by folate, vitamin B 12 , and methylenetetrahyfrofolate reductase (MTHFR) C677T gene polymorphism. Between December 1, 2017 and January 5, 2018, we conducted a panel study in 88 young college students in Guangzhou, China, and received 5 rounds of health examinations. Real-time concentrations of PMs with aerodynamic diameter ≤2.5 (PM 2.5), ≤1.0 (PM 1.0), and ≤0.1 (PM 0.1) were monitored, and the serum HCY metabolism markers (i.e., HCY, S-Adenosylhomocysteine [SAH], and S-Adenosylmethionine [SAM]) were repeatedly measured. We applied linear mixed effect models combined with a distributed lag model to evaluate the associations of PMs with the HCY metabolism markers. We also explored effect modifications of folate, vitamin B 12 , and the MTHF R C677T polymorphism on the associations. We observed that higher concentrations of PM 2.5 and PM 1.0 were associated with higher serum levels of HCY, SAH, SAM, and SAM/SAH ratio (e.g., a 10 μg/m³ increase in PM 2.5 during lag 0 day and lag 5 day was significantly associated with 1.3–19.4%, 1.3–28.2%, 6.2–64.4%, and 4.8–28.2% increase in HCY, SAH, SAM, and SAM/SAH ratio, respectively). In addition, we observed that the associations of PM 2.5 with the HCY metabolism markers were stronger in participants with lower B vitamins levels. This study demonstrated that short-term exposure to PM 2.5 and PM 1.0 was deleteriously associated with the HCY metabolism markers, especially in people with lower B vitamins levels. Image 1 • PM 2.5 and PM 1.0 exposures were associated with HCY metabolism markers. • B vitamins modified the associations of PMs with HCY metabolism markers. • MTHFR genotype did not modify the associations of PMs with HCY metabolism markers. Short-term exposures to PM 2.5 and PM 1.0 were deleteriously associated with the HCY metabolism markers, and the associations were modified by B vitamins. [ABSTRACT FROM AUTHOR]