The novel intervention effect of cold green tea beverage on high-fat diet induced obesity in mice.

• Cold green tea beverage contained considerable catechins, gallic acid and theanine. • Cold green tea beverage could significantly prevent obesity. • The anti-obesity effects might be attributed to the stimulation of serum peptide YY (PYY) secretion. • Cold green tea beverage could modulate some specific bacteria. This study was designed to explore the anti-obesity effects of cold-water brewed green tea (CWB-GT) on high-fat diet (HFD)-fed mice. CWB-GT was prepared by brewing tea leaves with 30 ℃ cold water, which contained considerable active ingredients including catechins, gallic acid and theanine. Our data demonstrated that CWB-GT could significantly ameliorate obesity and modulate gut microbiota composition of HFD-fed mice by affecting some specific bacteria. Besides, the expression of lipogenesis-related genes including FAS, HMGR and SREBP-1c in liver was inhibited and the expression of fatty acid oxidation-related genes including PPARα and PGC-1α in adipose tissue was enhanced. These benefits might be attributed to the stimulation of serum peptide YY (PYY) secretion. In particular, specific bacteria modulated by CWB-GT including Lachnospiraceae bacterium DW67, Blautia coccoides, Parabacteroides merdae and Bacteroides_vulgatus might be key mediators in exerting anti-obesity effects. This study suggested that CWB-GT could represent a novel functional low-calorie beverage. [ABSTRACT FROM AUTHOR]