MiR-376a-3p alleviates the development of glioma through negatively regulating KLF15.

OBJECTIVE: The purpose of this study was to uncover the role of microRNA- 376a-3p (miR-376a-3p) in mediating migratory and invasive capacities of glioma, as well as the underlying mechanism. PATIENTS AND METHODS: MiR-376a-3p levels in 39 collected glioma tissues were detected. After collecting clinical data of included glioma patients, the relationship between miR- 376a-3p level and clinical features of glioma was analyzed. Next, regulatory effects of miR-376a- 3p on proliferative and metastatic capacities of U251 and T98-G cells were assessed. Downstream genes of miR-376a-3p were searched by bioinformatics approach. At last, the involvement of KLF15 in the development of glioma regulated by miR-376a-3p was explored. RESULTS: It was found that miR-376a-3p was lowly expressed in glioma tissues. Low level of miR-376a-3p was linked to high metastasis rate and poor prognosis in glioma. Besides, overexpression of miR-376a-3p suppressed proliferative and metastatic capacities of glioma cells. KLF15, the downstream gene binding miR-376a-3p, was highly expressed in glioma, and displayed a negative correlation to miR-376a-3p. Notably, KLF15 was able to abolish the regulatory effects of miR- 376a-3p on phenotypes of glioma cells. CONCLUSIONS: MiR-376a-3p is related to lymphatic metastasis and distant metastasis of glioma, and alleviates metastasis of glioma by negatively regulating KLF15. [ABSTRACT FROM AUTHOR]