Dose-response analysis of toxic effect from palm oil mill effluent (POME) by-products on biohydrogen producing bacteria – A preliminary study on microbial density and determination of EC50.

The stimulant and toxicity effects of reported organic (acetic acid, propionic acid, butyric acid, formic acid, oil & grease) and inorganic (copper) by-products presented in palm oil mill effluent on anaerobic bacterial population were examined in this paper. The toxicity test had shown that acetic, propionic and butyric acids tend to stimulate the bacterial density level (survival rate more than 50%), while formic acid, copper, oil and grease were shown to have suppressed the density level (survival rate less than 50%). The highest biomass recorded was 1.66 mg/L for the concentration of acetic acid at 216 mg/L and lowest biomass concentration, 0.90 mg/L for copper at 1.40 mg/L. Biohydrogen-producing bacteria have a favourable growth rate around pH 5.5. The comparison of half maximal effective concentration (EC 50) values between two test duration on the effects of organic and inorganic by-products postulate that bacteria had a higher tolerance towards volatile fatty acids. While acetic, butyric and propionic acids had exhibited higher tolerance EC 50 values for bacteria, but the opposite trend was observed for formic acid, copper and oil & grease. Image 1 • Acetic, butyric and propionic acid were proven to elevate bacterial biomass concentration. • Formic acid, copper, oil and grease have the ability to arrest bacterial growth. • Optimum pH for mixed culture anaerobic bacteria growth was mildly acidic. [ABSTRACT FROM AUTHOR]