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Translocation of lysophosphatidic acid phosphatase in response to gonadotropin-releasing hormone to the plasma membrane in ovarian cancer cell.
- Source :
- American Journal of Obstetrics & Gynecology; Jul2004, Vol. 191 Issue 1, p143-149, 7p, 1 Chart, 2 Graphs
- Publication Year :
- 2004
-
Abstract
- Objective: Lysophosphatidic acid mediates proliferative and/or morphologic effects on multiple cell lineages, which include ovarian cancer cells. Lysophosphatidic acid hydrolysis limits the duration of lysophosphatidic acid's action. We examined hormonal translocation of lipid phosphate phosphatase type 3 to the plasma membrane in gonadotropin-releasing hormone-responsive ovarian cancers. Study design: Ovarian cancers that were removed surgically and the ovarian cancer cell lines Caov-3 and SK-OV-3 were examined. Lipid phosphate phosphatase type 3 protein and activity in plasma membranes were assessed by immunohistochemical staining with lipid phosphate phosphatase type 3-specific antibodies and by the measurement of the conversion of exogenous [<superscript>3</superscript>H- oleoyl]lysophosphatidic acid to mono[<superscript>3</superscript>H-oleoyl]glycerol, respectively. Results: In ovarian cancers that were removed surgically, the cell surface staining and activity measurements indicated that a portion of the enzyme was localized to the plasma membrane. In Caov-3 cells and SK-OV-3 cells, lipid phosphate phosphatase type 3 protein was present both in the cytoplasm and at the plasma membrane. Treatment of the cells with a gonadotropin-releasing hormone agonist buserelin produced a rapid and progressive translocation of lipid phosphate phosphatase type 3 protein to the plasma membrane, with a concomitant loss of cytoplasmic staining. The enzyme activity in plasma membrane was also increased when the cell lines were exposed to the gonadotropin-releasing hormone agonist in intact cells before the assay of the cell membranes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00029378
- Volume :
- 191
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- American Journal of Obstetrics & Gynecology
- Publication Type :
- Academic Journal
- Accession number :
- 14309400
- Full Text :
- https://doi.org/10.1016/j.ajog.2004.01.038