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Overexpression of miRNA-410-3p protects hypoxia-induced cardiomyocyte injury via targeting TRAF5.
- Source :
- European Review for Medical & Pharmacological Sciences; 2019, Vol. 23 Issue 20, p9050-9057, 8p
- Publication Year :
- 2019
-
Abstract
- OBJECTIVE: This study aims to clarify the influence of microRNA-410-3p (miRNA-410-3p) on hypoxia-induced injury in cardiomyocytes. MATERIALS AND METHODS: MiRNA-410-3p level, apoptotic rate, and cell viability in AC16 cells undergoing normoxia or hypoxia preconditioning were assessed. The regulatory effects of miRNA-410-3p and TRAF5 on the proliferative and apoptotic abilities of AC16 cells were evaluated. The binding relationship between miRNA-410-3p and TRAF5 was verified by Dual-Luciferase Reporter Gene Assay. RESULTS: Hypoxia preconditioning triggered apoptosis and inhibited the viability in AC16 cells. MiRNA-410-3p was downregulated in cardiomyocytes under the hypoxic environment. The overexpression of miRNA-410-3p stimulated proliferation and inhibited apoptosis in hypoxia preconditioning AC16 cells. TRAF5 was proved to be the target of miRNA-410-3p. TRAF5 level was negatively regulated by miRNA-410-3p. The silence of TRAF5 could reverse viability and apoptosis changes in hypoxic AC16 cells overexpressing miRNA-410-3p. CONCLUSIONS: M iRNA-410-3p p rotects hypoxia-induced proliferation suppression and apoptosis stimulation in cardiomyocytes via targeting TRAF5. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11283602
- Volume :
- 23
- Issue :
- 20
- Database :
- Supplemental Index
- Journal :
- European Review for Medical & Pharmacological Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 139487394