Aqueous extract of Houttuynia cordata ameliorates aortic endothelial injury during hyperlipidemia via FoxO1 and p38 MAPK pathway.

• In vivo, HAE alleviated hyperlipemia-induced endothelial and mitochondrial dysfunction of thoracic aorta. • In vitro, HAE ameliorated free fatty acid-induced endothelial impairment and mitochondrial dysfunction. • HAE ameliorated mitochondrial biogenesis by activating FoxO1, which confirmed the in vivo findings. • The anti-inflammatory response of HAE was mainly regulated via p38 MAPK pathway. Increasing evidence demonstrates that hyperlipidemia plays a causal role in the development of cardiovascular diseases (CVDs) due to endothelial dysfunction. Houttuynia cordata aqueous extract (HAE), using as both medicine and food, whose effect and mechanism were investigated in endothelial dysfunction. In vivo , it profoundly attenuated hyperlipidemia-induced accumulation of serum metabolites, which in turn contributed to alleviating morphologic and functional damage in vascular endothelium. Moreover, the decrease expression of FoxO1/PGC-1α and mitochondrial complexes in thoracic vessels were ameliorated by HAE administration. Through in vitro cultures, consistently, the results showed that HAE predominantly activated PGC-1α for mitochondrial biogenesis via enhancing FoxO1 expression. Additionally, silencing of FoxO1 remarkably abolished the ability of HAE to augment PGC-1α expression, implying the imperative role of FoxO1 during protection. Besides, the anti-inflammation was mainly mediated via p38 MAPK pathway. Briefly, our study demonstrated that HAE ameliorated hyperlipidemia-induced endothelial injury via upregulating FoxO1/PGC-1α and suppressing p38 MAPK. [ABSTRACT FROM AUTHOR]