Cytotoxicity Test of Sponge Amnion on BHK-21 Fibroblast Cell.

Biomaterials that can be used to accelerate wound healing is the amniotic membrane that contains growth hormone. In its use, amniotic membrane tends to be difficult to use because of their sheets form. To make it easier to use, the amniotic membrane is mixed with gelatin as an adhesive material to produce sponge amnion. Sponge amnion which is used as a biomaterial needs to meet several requirements, one of them is biocompatibility. To complete these requirements cytotoxicity testing is necessary to determine the toxic potential that a material may produce. The cytotoxicity test was performed on BHK-21 fibroblast cells. The aim of this study is to determine the citotoxicity effect of amnion in sponge form on BHK-21 fibroblast cells. This study was done towards the culture cell line BHK-21 using MTT assay method. In this study used four sample groups. Two groups are the control group (media control and cell control), and the other two groups are samples consisted of amniotic membrane and sponge amnion. Living cells were quantified after treatment by ELISA reader on 620 nm. The fibroblast cell life percentage after exposure with amniotic membrane and sponge amnion is 98,16% and 98.13%. Based on the ANOVA test result was obtained P-value of 0.000 (P-value <0.05) so that it can be said that the treatment result gives a significant difference. Amnion in sponge form has no toxic for BHK-21 fibroblast cell. [ABSTRACT FROM AUTHOR]