Size- and shape-dependent toxicity of silver nanomaterials in green alga Chlorococcum infusionum.

Abstract Silver nanomaterials (AgNMs) of different shapes and sizes are potentially toxic to aquatic organisms. However, studies on the toxicity of AgNMs and on their shape-dependent effects on algae are scarce. The present study evaluated the effects of three AgNMs (silver nanospheres, AgNPs; silver nanowires, AgNWs; silver nanoplates, AgPLs) with different shapes coated with polyvinylpyrrolidone on the growth and photosynthetic performance of an alga, Chlorococcum infusionum. We used growth measurements and determined the photosynthetic parameters based on chlorophyll fluorescence transients in the algal cells exposed to different concentrations of the three AgNMs. The effective concentrations at 50% (EC 50) of AgNPs, AgNWs, and AgPLs were calculated to be 0.1, 0.045, and 0.021 mg/L, respectively. The results showed that the toxicity of AgNMs in C. infusionum was in the order, AgPLs (40 nm diameter) > AgNWs (21,000 nm length × 42 nm diameter) > AgNPs (57 nm diameter), based on the decrease in growth and three photosynthetic activities. We propose that the toxic potential of AgNMs is primarily dependent on their diameter and secondarily on their shape. Overall, this study provides, for the first time, a comparison of the growth and photosynthetic activities of C. infusionum exposed to AgNMs of three different shapes. Graphical abstract fx1 Highlights • Effect of shape on silver nanomaterial toxicity on algal species is limited. • Comparative toxicity of silver nanomaterials of different shapes on Chlorococcum infusionum was evaluated. • Toxicity of the silver nanomaterials in C. infusionum was in the sequence AgPLs > AgNWs > AgNPs. • Toxic potential of silver nanomaterials is primarily dependent on their diameter and secondarily on their shape. [ABSTRACT FROM AUTHOR]