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A simple method for rapid screening of biosurfactant-producing strains using bromothymol blue alone.
- Source :
- Biocatalysis & Agricultural Biotechnology; Oct2018, Vol. 16, p121-125, 5p
- Publication Year :
- 2018
-
Abstract
- Abstract Biosurfactants are deemed as green replacements of synthetic surfactants. Lipopeptides, a class of biosurfactants, have excellent surface active properties along with anti-microbial and anti-tumor activities. Identifying microorganisms producing high titre of lipopeptide is of great importance in reducing production cost as currently lipopeptide production is not commercially competitive. A quantitative colorimetric assay method was developed for detecting lipopeptides and high-throughput screening of lipopeptide-producing strains by mixing bromothymol blue solution (pH 7.2) with lipopeptide-containing samples resulting in an instantaneous and stable color change. This color change is quantified spectrophotometrically at 410 nm and 616 nm showing linearly quantitative response to increased concentrations of 3 major lipopeptides (surfactin, iturin and fengycin) at 0–1 g/l. The assay was used on both cell-free broth and crude extracts of lipopeptide-producing strains and the titre obtained from the assay was supported by HPLC data. This assay can be used for screening lipopeptide-producing strains and quantitatively detecting lipopeptides either in purified form or in cell-free broth. Graphical abstract fx1 Highlights • A very simple method for rapid detection of biosurfactants. • No pre-incubation of samples is required. • Suitable for quantitative assay of lipopeptides and other surfactants. • Suitable for high throughput screening of biosurfactant-producing strains. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOSURFACTANTS
THYMOL
ANTI-infective agents
ANTINEOPLASTIC agents
PEPTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 18788181
- Volume :
- 16
- Database :
- Supplemental Index
- Journal :
- Biocatalysis & Agricultural Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 133093047
- Full Text :
- https://doi.org/10.1016/j.bcab.2018.07.027