The Differences Scaffold Composition in Pore Size and Hydrophobicity Properties as Bone Regeneration Biomaterial.

Surgical procedure using bone replacement materials are still needed to accelerate new bone formation. Tissue engineering concept develop the research in scaffold biomaterial. Chitosan, gelatin and bovine hydroxyapatite combination can be synthesized as an ideal scaffold biomaterial that has a biomimetic properties of bone tissue. To determine pore size and hydrophobicity properties of chitosan-gelatin/bovine hydroxyapatite scaffold at various eligible ratios in bone tissue engineering. Scaffold chitosan-gelatin/bovine hydroxyapatite with a ratio of 20:80, 30:70 and 40:60 synthesized using freeze dry method. Scaffold on each ratio was tested by pore size examination using Scanning Electron Microscope. The ratio of swelling and water content percentage was done by measuring the initial weight and final weight after being soaked in distillate water for 1, 3 and 7 days. The smallest pore size was obtained at a 20:80 ratio scaffold with a mean value of 254.44 ± 37.96 µm and the largest on a 40:60 ratio scaffold with a mean value of 423.04 ± 68.72 µm. Swelling ratios and water content percentage were highest on the chitosan-gelatin/bovine hydroxyapatite ratio of 40:60 at day 7 (2,904 ± 0.531 and 75.84 ± 2.6%). The pore size and hydrophobicity properties corresponding to bone tissue regeneration biomaterials were obtained on the 20:80 and 30:70 ratios chitosan-gelatin/bovine hydroxyapatite scaffold. [ABSTRACT FROM AUTHOR]