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miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN.
- Source :
- Biomedical & Environmental Sciences; Mar2018, Vol. 31 Issue 3, p171-185, 15p
- Publication Year :
- 2018
-
Abstract
- Objective To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes. Methods The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting. Results Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1. Conclusion Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- ESOPHAGEAL cancer
CANCER cell proliferation
EXOSOMES
CELL communication
PTEN protein
Subjects
Details
- Language :
- English
- ISSN :
- 08953988
- Volume :
- 31
- Issue :
- 3
- Database :
- Supplemental Index
- Journal :
- Biomedical & Environmental Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 129386421
- Full Text :
- https://doi.org/10.3967/bes2018.023