Back to Search
Start Over
Expression profiling of immune genes in classical swine fever vaccinated indigenous and crossbred piglets.
- Source :
- Indian Journal of Animal Sciences; 2017, Vol. 87 Issue 10, p1184-1189, 6p
- Publication Year :
- 2017
-
Abstract
- Classical swine fever is a highly contagious disease of pigs which courses from life-threatening to asymptomatic, depending on the virulence of the virus strain and the immune-competence of the host. The present study was undertaken to investigate the expression of immunologically important genes, viz. IFNα, IFNβ, SLA, SLA-2, SLA-DR, Ii, SLA-DM, CSK and JUN and to ascertain genetic group differences on the basis of humoral immune response. Blood samples were collected from 5 indigenous and 6 crossbred piglets at pre-vaccination and after 28<superscript>th</superscript> day of classical swine fever (CSF) vaccination. On 28<superscript>th</superscript> day, the competitive Enzyme Linked Immunosorbent Assay (cELISA) revealed poor humoral immune response (E2 antibodies) in indigenous piglets (84.80%) as compared to crossbred piglets (98.33%) in response to CSF vaccination. The expression level of genes was analyzed in three ways, viz. indigenous 28<superscript>th</superscript> day post-vaccination (28dpv) versus pre-vaccination, crossbred 28<superscript>th</superscript> day post-vaccination versus pre-vaccination and crossbred 28<superscript>th</superscript> day post-vaccination versus indigenous 28<superscript>th</superscript> day post-vaccination. The study showed that IFNα, IFNβ, SLA, SLA-2, Ii, SLA-DM, CSK and JUN were significantly upregulated in crossbred piglets than indigenous piglets at 28<superscript>th</superscript> day post-vaccination. But the SLA-DR was significantly downregulated in CSF vaccinated crossbred over indigenous piglets. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03678318
- Volume :
- 87
- Issue :
- 10
- Database :
- Supplemental Index
- Journal :
- Indian Journal of Animal Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 126129785
- Full Text :
- https://doi.org/10.56093/ijans.v87i10.75238