Assessing the toxicity of triphenyltin to different life stages of the marine medaka Oryzias melastigma through a series of life-cycle based experiments.

Toxic effects of triphenyltin (TPT) to different life stages of the marine medaka Oryzias melastigma were investigated through a series of life-cycle based exposure experiments. In embryo stage, TPT exposure could elevate the heartbeat rate at Day 6–8 post-fertilization and increase the expression levels of five heart development related genes (i.e., ATPase , COX2 , BMP4 , GATA4 and NKX2.5 ). In larval stage, TPT shortened the body length at ≥ 10 μg/L and suppressed the swimming activity of the fish larvae at Day 1 post-hatching at 50 μg/L. In reproductive stage, TPT exposure resulted in a male-biased sex ratio (2 μg/L) and reduced the gonadosomatic index (GSI) in females (≥ 0.1 μg/L), which might in turn lead to a decline in their population fitness. The reproductive stage of O. melastigma was more sensitive to TPT than other stages, while the GSI of female medaka was the most sensitive endpoint. [ABSTRACT FROM AUTHOR]