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Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance.

Authors :
Hosseinkhani, Baharak
van den Akker, Nynke
D'Haen, Jan
Gagliardi, Mick
Struys, Tom
Lambrichts, Ivo
Waltenberger, Johannes
Nelissen, Inge
Hooyberghs, Jef
Molin, Daniel G.M.
Michiels, Luc
Source :
Nanomedicine: Nanotechnology, Biology & Medicine; Jul2017, Vol. 13 Issue 5, p1663-1671, 9p
Publication Year :
2017

Abstract

A major conceptual breakthrough in cell signaling has been the finding of EV as new biomarker shuttles in body fluids. Now, one of the major challenges in using these nanometer-sized biological entities as diagnostic marker is the development of translational methodologies to profile them. SPR offers a promising label-free and real time platform with a high potential for biomarker detection. Therefore, we aimed to develop a uniform SPR methodology to detect specific surface markers on EV derived from patient with CHD. EVs having an approximate size range between 30 and 100 nm (~48.5%) and 100-300 nm (~51.5%) were successfully isolated. The biomarker profile of EV was verified using immunogold labeling, ELISA and SPR. Using SPR, we demonstrated an increased binding of EV derived from patients with CHD to anti-ICAM-1 antibodies as compared to EV from healthy donors. Our current findings open up novel opportunities for in-depth and label-free investigation of EV. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
15499634
Volume :
13
Issue :
5
Database :
Supplemental Index
Journal :
Nanomedicine: Nanotechnology, Biology & Medicine
Publication Type :
Academic Journal
Accession number :
123696136
Full Text :
https://doi.org/10.1016/j.nano.2017.03.010