Effects of alginate oligomer on the expression of cell cycle- and stress-related genes in Chlamydomonas reinhardtii.

Enzymatically prepared alginate oligomer (AO) promoted the growth ofChlamydomonas reinhardtiiin a concentration-dependent manner. AO at 2.5 mg/mL induced increase in expression levels of cyclin A, cyclin B, and cyclin D inC. reinhardtii. CuSO4at 100 μM suppressed the growth ofC. reinhardtiin, and AO at 2.5 mg/mL significantly alleviated the toxicity of CuSO4. Increased intracellular reactive oxygen species level inC. reinhardtiiinduced by CuSO4was reduced by AO. After cultivation with CuSO4at 100 μM, expression levels of ascorbate peroxidase and superoxide dismutase inC. reinhardtiiwere increased, and AO reduced the increased levels of these enzymes. These results suggest that AO exhibits beneficial effects onC. reinhardtiithrough influencing the expression of various genes not only at normal growth condition but also under CuSO4stress. [ABSTRACT FROM PUBLISHER]