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Transforming growth factor beta 1 increases collagen content, and stimulates procollagen I and tissue inhibitor of metalloproteinase-1 production of dental pulp cells: Role of MEK/ERK and activin receptor-like kinase-5/Smad signaling.

Authors :
Lin, Po-Shuen
Chang, Hsiao-Hua
Yeh, Chien-Yang
Chang, Mei-Chi
Chan, Chiu-Po
Kuo, Han-Yueh
Liu, Hsin-Cheng
Liao, Wan-Chuen
Jeng, Po-Yuan
Yeung, Sin-Yuet
Jeng, Jiiang-Huei
Source :
Journal of the Formosan Medical Association; May2017, Vol. 116 Issue 5, p351-358, 8p
Publication Year :
2017

Abstract

<bold>Background/purpose: </bold>In order to clarify the role of transforming growth factor beta 1 (TGF-β1) in pulp repair/regeneration responses, we investigated the differential signaling pathways responsible for the effects of TGF-β1 on collagen turnover, matrix metalloproteinase-3 (MMP-3), and tissue inhibitor of metalloproteinase-1 (TIMP-1) production in human dental pulp cells.<bold>Methods: </bold>Pulp cells were exposed to TGF-β1 with/without pretreatment and coincubation by 1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenyl mercapto)butadiene (U0126; a mitogen-activated protein kinase kinase [MEK]/extracellular signal-regulated kinase [ERK] inhibitor) and 4-(5-benzol[1,3]dioxol-5-yl-4-pyrldin-2-yl-1H- imidazol-2-yl)-benzamide hydrate (SB431542; an activin receptor-like kinase-5/Smad signaling inhibitor). Sircol collagen assay was used to measure cellular collagen content. Culture medium procollagen I, TIMP-1, and MMP-3 levels were determined by enzyme-linked immunosorbent assay.<bold>Results: </bold>TGF-β1 increased the collagen content, procollagen I, and TIMP-1 production, but slightly decreased MMP-3 production of pulp cells. SB431542 and U0126 prevented the TGF-β1-induced increase of collagen content and TIMP-1 production of dental pulp cells.<bold>Conclusion: </bold>These results indicate that TGF-β1 may be involved in the healing/regeneration processes of dental pulp in response to injury by stimulation of collagen and TIMP-1 production. These events are associated with activin receptor-like kinase-5/Smad2/3 and MEK/ERK signaling. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09296646
Volume :
116
Issue :
5
Database :
Supplemental Index
Journal :
Journal of the Formosan Medical Association
Publication Type :
Academic Journal
Accession number :
122623583
Full Text :
https://doi.org/10.1016/j.jfma.2016.07.014