S _allele identification and genetic diversity analysis of apricot cultivars.

In this study, the sexual incompatibility andS-allele diversity of 24 Turkish apricot cultivars, Paviot and Sakıt-1 as parents and 127 F1progenies were identified by polymerase chain reaction (PCR) and sequencing techniques. Additionally, genetic diversity and relatedness among the 24 cultivars were determined using 18 simple sequence repeat (SSR) markers from the genusPrunus. PCR forS_alleles identified nine differentS-RNase alleles in the 24 apricot cultivars, namelySc,S1,S2,S8,S9,S20,S24,S52, andS53. All primers amplified only oneS_allele in the cultivars Adilcevaz-1, Adilcevaz-3, Ethembey, Pasamismisi, Canakkale, and Soganci. Most of the Turkish cultivars were self-incompatible. TheScallele was present in only three cultivars (Canakkale, Ethembey, Imrahor) that are, therefore, self-compatible. TheS_alleles of cultivars Paviot and Sakıt-1 displayed homology with theSc,S2andS20, andS52alleles. In the 127 F1genotypes, the twoS_alleles of Paviot were inherited by roughly half of the offspring, while about 76% of the offspring inherited theS52allele from Sakıt-1, and less than 24% inheritedS20. The amplification using all SSR 18 primers was successful and produced 128 polymorphic alleles with an average of 7.11 alleles per locus. Among the apricot cultivars studied, expected heterozygosity (He) ranged from 0.33 to 0.72, observed heterozygosity (Ho) ranged from 0.42 to 1.00, PIC values were between 0.28 and 0.89, and similarity rates were between 0.30 and 0.68. The cultivars Levent and Ozal were genetically closest (0.68) while cultivars Sakıt-3 and Sogancı were the most distinct (0.30). [ABSTRACT FROM AUTHOR]