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Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study.
- Source :
- Comparative Biochemistry & Physiology Part D: Genomics & Proteomics; Sep2016, Vol. 19, p78-89, 12p
- Publication Year :
- 2016
-
Abstract
- Marine invertebrate aestivation is a unique strategy for summer survival in response to hot marine conditions. The sea cucumber, Apostichopus japonicus , is an excellent model marine invertebrate for studies of environmentally-induced aestivation. In the present study, we used a tandem mass tag (TMT)-coupled LC-MS/MS approach to identify and quantify the global proteome expression profile over the aestivation-arousal cycle of A. japonicus . A total of 3920 proteins were identified from the intestine of sea cucumber. Among them, 630 proteins showed significant differential expression when comparing three conditions of sea cucumbers: non-aestivating (active), deep-aestivation (at least 15 days of continuous aestivation), and arousal after aestivation (renewed moving and feeding). Sea cucumbers in deep aestivation showed substantial differentially expressed proteins (143 up-regulated and 267 down-regulated proteins compared with non-aestivating controls). These differentially expressed proteins suggested that protein and phospholipid probably are major fuel sources during hypometabolism and a general attenuation of carbohydrate metabolism was observed during deep aestivation. Differentially expressed proteins also provided the first global picture of a shift in protein synthesis, protein folding, DNA binding, apoptosis, cellular transport and signaling, and cytoskeletal proteins during deep aestivation in sea cucumbers. A comparison of arousal from aestivation with deep aestivation, revealed a general reversal of the changes that occurred in aestivation for most proteins. Western blot detection further validated the significant up-regulation of HSP70 and down-regulation of methyltransferase-like protein 7A-like in deep-aestivation. Our results suggest that there is substantial post-transcriptional regulation of proteins during the aestivation-arousal cycle in sea cucumbers. [ABSTRACT FROM AUTHOR]
- Subjects :
- SEA cucumbers
PROTEOMICS
PHOSPHOLIPIDS
DNA-binding proteins
PROTEIN expression
Subjects
Details
- Language :
- English
- ISSN :
- 1744117X
- Volume :
- 19
- Database :
- Supplemental Index
- Journal :
- Comparative Biochemistry & Physiology Part D: Genomics & Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 117435987
- Full Text :
- https://doi.org/10.1016/j.cbd.2016.06.005