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Round robin test on quantification of amyloid-β 1–42 in cerebrospinal fluid by mass spectrometry.
- Source :
- Alzheimer's & Dementia: The Journal of the Alzheimer's Association; Jan2016, Vol. 12 Issue 1, p55-59, 5p
- Publication Year :
- 2016
-
Abstract
- Introduction Cerebrospinal fluid (CSF) amyloid-β 1–42 (Aβ 42 ) is an important biomarker for Alzheimer's disease, both in diagnostics and to monitor disease-modifying therapies. However, there is a great need for standardization of methods used for quantification. To overcome problems associated with immunoassays, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as a critical orthogonal alternative. Methods We compared results for CSF Aβ 42 quantification in a round robin study performed in four laboratories using similar sample preparation methods and LC-MS instrumentation. Results The LC-MS results showed excellent correlation between laboratories ( r 2 >0.98), high analytical precision, and good correlation with enzyme-linked immunosorbent assay ( r 2 >0.85). The use of a common reference sample further decreased interlaboratory variation. Discussion Our results indicate that LC-MS is suitable for absolute quantification of Aβ 42 in CSF and highlight the importance of developing a certified reference material. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15525260
- Volume :
- 12
- Issue :
- 1
- Database :
- Supplemental Index
- Journal :
- Alzheimer's & Dementia: The Journal of the Alzheimer's Association
- Publication Type :
- Academic Journal
- Accession number :
- 112134882
- Full Text :
- https://doi.org/10.1016/j.jalz.2015.06.1890