Effect of PDGF-Rb antagonist imatinib on endometrial injury repairing in mouse model.

Objective To study the effects of PDGF-Rb antagonists imatinib on endometrial injury repairing in the mouse model. Methods The cultured MSCs cells from male mice were marked with BrdU in vitro , and then transplanted to the female mice which suffered from radiation injury through tail vein, PDGF-Rb antagonists imatinib was injected through abdominal cavity. Four groups were arranged, which were radiation transplantation group, normal control group, imatinib intervention group and radiation control group. BrdU incorporation, SRY expression and MVD status were detected in uterus of mice. Results SRY gene was negative expressed in normal control group and radiation control group. SRY gene presented positive in radiation transplantation group and imatinib intervention group; BrdU incorporation showed negative in radiation control group and normal control group which died in the early stage in mice; the incorporation of BrdU was higher in radiation transplantation group compared with imatinib intervention group; CD34 was positive on the uterus of all the four groups, which showed highest in radiation control group and lowest in radiation control group; The MVD in imatinib intervention group was lower than radiation control group; the difference of MVD was significantly compared with normal control group ( P < 0.05). Conclusions PDGF-Rb antagonists imatinib could inhibit the repairing function of MSCs in the endometrial lesions in mice. [ABSTRACT FROM AUTHOR]