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Elevated expression of catechol-O-methyltransferase is associated with labor and increased prostaglandin E(2) production by human fetal membranes.
- Source :
- American Journal of Obstetrics & Gynecology; Nov2009, Vol. 201 Issue 5, p496.e1-7, 1p
- Publication Year :
- 2009
-
Abstract
- <bold>Objective: </bold>The purpose of this study was to evaluate the expression and function of catechol-O-methyltransferase in human fetal membranes at term.<bold>Study Design: </bold>Fetal membranes obtained from women between 38-42 weeks of gestation, after (1) vaginal delivery with spontaneous labor and (2) prelabor elective cesarean section (no labor), were assayed for catechol-O-methyltransferase expression using quantitative real-time polymerase chain reaction analysis, immunohistochemistry, and Western blot analysis. Prostaglandin E(2) secretion from amnion and choriodecidua explants treated with or without catechol-O-methyltransferase inhibitor was assayed by enzyme-linked immunosorbent analysis.<bold>Results: </bold>Amnion layer of fetal membranes from laboring women expressed significantly higher levels of catechol-O-methyltransferase, compared with those from women with no labor. Catechol-O-methyltransferase was higher in the amnion layer than in choriodecidua. Selective catechol-O-methyltransferase inhibition significantly decreased prostaglandin E(2) production from fetal membranes.<bold>Conclusion: </bold>Labor increases catechol-O-methyltransferase expression in the amnion of human fetal membranes. Selective catechol-O-methyltransferase inhibition decreased prostaglandin E(2) secretion in fetal explant cultures, suggesting a role for catechol-O-methyltransferase in human labor and delivery. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00029378
- Volume :
- 201
- Issue :
- 5
- Database :
- Supplemental Index
- Journal :
- American Journal of Obstetrics & Gynecology
- Publication Type :
- Academic Journal
- Accession number :
- 105236521
- Full Text :
- https://doi.org/10.1016/j.ajog.2009.05.041