Quantification of Circulating miRNAs by Droplet Digital PCR: Comparison of EvaGreen- and TaqMan-Based Chemistries.

The article presents a study that involves comparison of quantifying MircoRNA (miRNA) with the QX200 Droplet Digital Polymerase Chain Reaction (ddPCR) system when used with EvaGreen dye-based and TaqMan probe-based assays. The study reveals that both assays can be equally used with the ddPCR system to quantify circulating miRNAs in human plasma and serum. Also the study establishes the basis for using EvaGreen dye-based assays on a ddPCR system for quantifying circulating miRNA biomarkers.