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In-vitro binding assay study of 99mTc-flouroquinolones with E. coli, Salmonella and Ps. aeruginosa.

Authors :
Qadir, Muhammad Abdul
Wattoo, Feroza Hamid
Yaseen, Mehwish
Atta, Sadia
Wattoo, Muhammad Hamid Sarwar
Ahmad, Sheikh Asrar
Gulzar, Asad
Source :
Alexandria Journal of Medicine; Mar2015, Vol. 51 Issue 1, p47-52, 6p
Publication Year :
2015

Abstract

A simple methodology was developed to evaluate binding efficiency of antibiotic members of fluoroquinolones, namely ciprofloxacin, ofloxacin and enorfloxacin, complexed with 99m Tc, against Escherichia coli , Salmonella and Pseudomonas aeruginosa bacterial strains. Radioactivity in the pellet, tips supernatant and micro-centrifugation tubes was counted separately for 5 s in a sodium iodide well-counter with dedicated nuclear medicine software. The overall percentage activity of the live and killed bacteria was found in the range of 5–46% with different types of labelled quinolones and bacteria. Activity of the labelled enorfloxacin and ciprofloxacin indicated acceptable results for both live and killed E. coli and Ps. aeruginosa. However, ofloxacin was found to be moderate for all the live bacterial stains. This developed methodology has achieved more than 95% labelling efficiency of 99m Tc with derivatized quinolones and also the observed results indicated that these complexes may be used as an infection specific imaging agents. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20905068
Volume :
51
Issue :
1
Database :
Supplemental Index
Journal :
Alexandria Journal of Medicine
Publication Type :
Academic Journal
Accession number :
101503051
Full Text :
https://doi.org/10.1016/j.ajme.2014.09.004