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Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC.

Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC.

Authors :
Jean, Christian
Oliveira, Nidia M.M.
Intarapat, Sittipon
Fuet, Aurélie
Mazoyer, Clément
De Almeida, Irene
Trevers, Katherine
Boast, Sharon
Aubel, Pauline
Bertocchini, Federica
Stern, Claudio D.
Pain, Bertrand
Source :
Stem Cell Research; Jan2015, Vol. 14 Issue 1, p54-67, 14p
Publication Year :
2015

Abstract

Pluripotent Embryonic Stem cell (ESC) lines can be derived from a variety of sources. Mouse lines derived from the early blastocyst and from primordial germ cells (PGCs) can contribute to all somatic lineages and to the germ line, whereas cells from slightly later embryos (EpiSC) no longer contribute to the germ line. In chick, pluripotent ESCs can be obtained from PGCs and from early blastoderms. Established PGC lines and freshly isolated blastodermal cells (cBC) can contribute to both germinal and somatic lineages but established lines from the former (cESC) can only produce somatic cell types. For this reason, cESCs are often considered to be equivalent to mouse EpiSC. To define these cell types more rigorously, we have performed comparative microarray analysis to describe a transcriptomic profile specific for each cell type. This is validated by real time RT-PCR and in situ hybridisation. We find that both cES and cBC cells express classic pluripotency-related genes (including cPOUV/OCT4, NANOG, SOX2/3, KLF2 and SALL4), whereas expression of DAZL, DND1, DDX4 and PIWIL1 defines a molecular signature for germ cells. Surprisingly, contrary to the prevailing view, our results also suggest that cES cells resemble mouse ES cells more closely than mouse EpiSC. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
18735061
Volume :
14
Issue :
1
Database :
Supplemental Index
Journal :
Stem Cell Research
Publication Type :
Academic Journal
Accession number :
100703687
Full Text :
https://doi.org/10.1016/j.scr.2014.11.005