Atrazine and chlorpyrifos exposure induces liver autophagic response in common carp.

Under normal conditions, autophagy occurs at basal levels but can be induced rapidly in response to stress conditions and extracellular signals. Increasing experimental evidence indicates that the expression of autophagy-related genes play very important roles in toxicology. Atrazine (ATR) and chlorpyrifos (CPF) are the most common agrochemical in the freshwater ecosystems of the world. This study assessed the effects of ATR, CPF and combined ATR/CPF exposure on the liver of common carp. Carp were sampled after a 40-d exposure to ATR and CPF, individually or in combination, followed by a 40-d recovery to measure the mRNA and protein levels of autophagy-related genes in the liver. In addition, we also investigated the change in ultrastructure in the liver. The results revealed that the mRNA and protein levels of microtubule-associated protein 1 light chain 3 B (LC3B) and dynein were significantly induced in the treated groups compared to the solvent control group. Transmission electron microscope assays indicated that autolysosomes were observed in the exposure and recovery groups. These results indicated that ATR and CPF could induce autophagy in carp liver. To the best of our knowledge, this is the first report to study the autophagy effects caused by sub-chronic exposure to ATR, CPF and the ATR/CPF combination in common carp. The information presented in the present study may provide new insights into the mechanisms used by fish to adapt to stressful environments. [ABSTRACT FROM AUTHOR]