Anti-inflammatory effects of triterpenes and steroid compounds isolated from the stem bark of Hiptage benghalensis.

In this study, we investigated the anti-inflammatory effects of triterpenes and steroid derivative isolated from the stem bark of Hiptage benghalensis in LPS-stimulated RAW 264.7 macrophages. In the extraction and isolation section, the chloroform-soluble fraction of the stem bark of H. benghalensis led to the isolation of seven triterpenes and a steroid derivative. These compounds were characterized as alnus-5(10)-en-3β-yl acetate ( 1 ), oleanan-3-one ( 2 ), 3β-acetoxy-9β-bauer-7-en-6-one ( 3 ), lupeol ( 4 ), (24R)-24-propylcholesterol ( 5 ), alnus-5(10)-en-3β-ol ( 6 ), 3β-acetoxy-20-hydroxylupane ( 7 ), and betulonic acid ( 8 ) by spectroscopic methods. In vitro anti-inflammatory activity, compounds 1, 2, 5, 6, 7 , and 8 significantly inhibited the productions of NO and PGE 2 , and protein expressions of iNOS and COX-2 in LPS-stimulated RAW 264.7 macrophages. Compounds 1, 2, 7 , and 8 also significantly increased the protein expression of IκBα, and decreased the protein expression of p-p65 and transcriptional activity of NF-κB in LPS-stimulated RAW 264.7 macrophages. Taken together, the present study clearly demonstrates that compounds isolated from the stem bark of H. benghalensis exhibit anti-inflammatory activity through the suppression of NF-κB in LPS-stimulated RAW 264.7 macrophages. [ABSTRACT FROM AUTHOR]