Inhibition Effect of Disintegrin on Posterior Capsular Opacification in Rabbits.

Objective To investigate the efficacy and safety of disintegrin DTY29 on inhibiting the proliferation of rabbit lens epithelial cells (LECs) in vitro and in vivo. Methods (l) In vitro: 36-well plates were divided into control group (a) and experimental groups (b, c and d), nine holes each group. The human LECs were cultured and scratched, and then culture media, 0.08 µmol/L, 0.4 µmol/L, and 2 µmol/L DTY29 were added into 4 groups, respectively. Scratch widths were photographed and measured at 0 h, 6h, 12h and 24 h. (2) In vivo: 36 male New Zealand white rabbits were randomly divided into control group (A), experimental groups (B, C and D), nine rabbits each group. Amounts of corneal endothelial cells were calculated before operative. Phacoemulsification was performed in all right eyes of the rabbits, and then 0.2 mL of Ringer's, 10 µ mol/L, 20 µmol/L and 40 µmol/L DTY29 solution was injected into the anterior chamber of groups A, B, C and D, respectively. Cornea, anterior chamber, pupil, posterior capsular opacification (PCO), and fundus were observed postoperatively. Results At6h, 12 h and 24 h of cells scratch test in vitro, the scratch widths of group a reduced significantly (P < 0.05) compared with groups b, c and d. There were no significant differences in corneal endothelial cells loss among groups A, B and C (P > 0.05), while a significant reduction was found in group D compared with groups A,B and C (< 0.05). PCO was observed in both groups A and B after 1-3 months postoperatively, but there was no significant difference in incidence (> 0.05). PCO hadn't occurred in groups C and D. Retinal detachment,choroidal detachment and other serious complications were not observed. Conclusion Disintegrin DTY29 could inhibit lens epithelial cells proliferation in vitro or in vivo,and there was a positive correlation between inhibiting effect and concentration. In vivo, corneal endothelial cells can be destroyed by DTY29 with a high concentration, and no side effects on intraocular tissues have been observed, which demonstrated the safety at a low concentration. The experiment results suggested that the optimum concentration ranged from 10 µmol/L to 20 µmol/L,and further investigation should be done to prove its safety and optimum concentration. [ABSTRACT FROM AUTHOR]