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Natural genetic variation impacts expression levels of coding, non-coding, and antisense transcripts in fission yeast.
- Source :
- Molecular Systems Biology; Nov2014, Vol. 10 Issue 11, pn/a-N.PAG, 23p
- Publication Year :
- 2014
-
Abstract
- Our current understanding of how natural genetic variation affects gene expression beyond well-annotated coding genes is still limited. The use of deep sequencing technologies for the study of expression quantitative trait loci ( eQTLs) has the potential to close this gap. Here, we generated the first recombinant strain library for fission yeast and conducted an RNA-seq-based QTL study of the coding, non-coding, and antisense transcriptomes. We show that the frequency of distal effects ( trans- eQTLs) greatly exceeds the number of local effects ( cis- eQTLs) and that non-coding RNAs are as likely to be affected by eQTLs as protein-coding RNAs. We identified a genetic variation of swc5 that modifies the levels of 871 RNAs, with effects on both sense and antisense transcription, and show that this effect most likely goes through a compromised deposition of the histone variant H2A.Z. The strains, methods, and datasets generated here provide a rich resource for future studies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17444292
- Volume :
- 10
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Molecular Systems Biology
- Publication Type :
- Academic Journal
- Accession number :
- 99707282
- Full Text :
- https://doi.org/10.15252/msb.20145123