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Using Bio-Functionalized Magnetic Nanoparticles and Dynamic Nuclear Magnetic Resonance to Characterize the Time-Dependent Spin-Spin Relaxation Time for Sensitive Bio-Detection.

Authors :
Shu-Hsien Liao
Kuen-Lin Chen
Chun-Ming Wang
Jen-Jie Chieh
Herng-Er Horng
Li-Min Wang
Wu, C. H.
Hong-Chang Yang
Source :
Sensors (14248220); 2014, Vol. 14 Issue 11, p21409-21417, 9p
Publication Year :
2014

Abstract

In this work, we report the use of bio-functionalized magnetic nanoparticles (BMNs) and dynamic magnetic resonance (DMR) to characterize the time-dependent spin-spin relaxation time for sensitive bio-detection. The biomarkers are the human C-reactive protein (CRP) while the BMNs are the anti-CRP bound onto dextran-coated Fe<subscript>3</subscript>O<subscript>4</subscript> particles labeled as Fe3O<subscript>4</subscript>-antiCRP. It was found the time-dependent spin-spin relaxation time, T<subscript>2</subscript>, of protons decreases as time evolves. Additionally, the ΔT<subscript>2</subscript> of of protons in BMNs increases as the concentration of CRP increases. We attribute these to the formation of the magnetic clusters that deteriorate the field homogeneity of nearby protons. A sensitivity better than 0.1 µg/mL for assaying CRP is achieved, which is much higher than that required by the clinical criteria (0.5 mg/dL). The present MR-detection platform shows promise for further use in detecting tumors, viruses, and proteins. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14248220
Volume :
14
Issue :
11
Database :
Complementary Index
Journal :
Sensors (14248220)
Publication Type :
Academic Journal
Accession number :
99660309
Full Text :
https://doi.org/10.3390/s141121409