Back to Search Start Over

GlycoDigest: a tool for the targeted use of exoglycosidase digestions in glycan structure determination.

Authors :
Gotz, Lou
Abrahams, Jodie L.
Mariethoz, Julien
Rudd, Pauline M.
Karlsson, Niclas G.
Packer, Nicolle H.
Campbell, Matthew P.
Lisacek, Frederique
Source :
Bioinformatics; Nov2014, Vol. 30 Issue 21, p3131-3133, 3p
Publication Year :
2014

Abstract

Summary: Sequencing oligosaccharides by exoglycosidases, either sequentially or in an array format, is a powerful tool to unambiguously determine the structure of complex N- and O-link glycans. Here, we introduce GlycoDigest, a tool that simulates exoglycosidase digestion, based on controlled rules acquired from expert knowledge and experimental evidence available in GlycoBase. The tool allows the targeted design of glycosidase enzyme mixtures by allowing researchers to model the action of exoglycosidases, thereby validating and improving the efficiency and accuracy of glycan analysis.Availability and implementation: http://www.glycodigest.org.Contact: matthew.campbell@mq.edu.au or frederique.lisacek@isb-sib.ch [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13674803
Volume :
30
Issue :
21
Database :
Complementary Index
Journal :
Bioinformatics
Publication Type :
Academic Journal
Accession number :
99224087
Full Text :
https://doi.org/10.1093/bioinformatics/btu425