Back to Search
Start Over
Covalently tethered TGF- β1 with encapsulated chondrocytes in a PEG hydrogel system enhances extracellular matrix production.
- Source :
- Journal of Biomedical Materials Research, Part A; Dec2014, Vol. 102 Issue 12, p4464-4472, 9p
- Publication Year :
- 2014
-
Abstract
- Healing articular cartilage defects remains a significant clinical challenge because of its limited capacity for self-repair. While delivery of autologous chondrocytes to cartilage defects has received growing interest, combining cell-based therapies with growth factor delivery that can locally signal cells and promote their function is often advantageous. We have previously shown that PEG thiol-ene hydrogels permit covalent attachment of growth factors. However, it is not well known if embedded chondrocytes respond to tethered signals over a long period. Here, chondrocytes were encapsulated in PEG hydrogels functionalized with transforming growth factor-beta 1 (TGF-β1) with the goal of increasing proliferation and matrix production. Tethered TGF-β1 was found to be distributed homogenously throughout the gel, and its bioactivity was confirmed with a TGF-β1 responsive reporter cell line. Relative to solubly delivered TGF-β1, chondrocytes presented with immobilized TGF-β1 showed significantly increased DNA content, and GAG and collagen production over 28 days, while maintaining markers of articular cartilage. These results indicate the potential of thiol-ene chemistry to covalently conjugate TGF-β1 to PEG to locally influence chondrocyte function over 4 weeks. Scaffolds with other or multiple tethered growth factors may prove broadly useful in the design of chondrocyte delivery vehicles for cartilage tissue engineering applications. © 2014 The Authors. Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc.: 102A: 4464-4472, 2014. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15493296
- Volume :
- 102
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Journal of Biomedical Materials Research, Part A
- Publication Type :
- Academic Journal
- Accession number :
- 98979809
- Full Text :
- https://doi.org/10.1002/jbm.a.35115