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Novel Design for Centrifugal Counter-Current Chromatography: VI. Ellipsoid Column.

Authors :
Gu, Dongyu
Yang, Yi
Xin, Xuelei
Aisa, HajiAkber
Ito, Yoichiro
Source :
Journal of Liquid Chromatography & Related Technologies; 2015, Vol. 38 Issue 1, p68-73, 6p, 1 Black and White Photograph, 2 Diagrams, 1 Chart, 5 Graphs
Publication Year :
2015

Abstract

A novel ellipsoid column was designed for centrifugal counter-current chromatography. Performance of the ellipsoid column with a capacity of 3.4 mL was examined with three different solvent systems composed of 1-butanol–acetic acid–water (4:1:5,v/v/v) (BAW), hexane–ethyl acetate–methanol-0.1 M HCl (1:1:1:1,v/v/v/v) (HEMH), and 12.5% (w/w) PEG1000 and 12.5% (w/w) dibasic potassium phosphate in water (PEG-DPP) each with suitable test samples. In dipeptide separation with BAW system, both stationary phase retention (Sf) and peak resolution (Rs) of the ellipsoid column were much higher at 0° column angle (column axis parallel to the centrifugal force) than at 90° column angle (column axis perpendicular to the centrifugal force), where elution with the lower phase at a low flow rate produced the best separation yielding Rs at 2.02 with 27.8% Sf at a flow rate of 0.07 mL/min. In the DNP-amino acid separation with HEMW system, the best results were obtained at a flow rate of 0.05 ml/min with 31.6% Sf yielding high Rs values at 2.16 between DNP-DL-glu and DNP-β-ala peaks and 1.81 between DNP-β-ala and DNP-L-ala peaks. In protein separation with PEG-DPP system, lysozyme and myolobin were resolved at Rs of 1.08 at a flow rate of 0.03 mL/min with 38.9% Sf. Most of those Rs values exceed those obtained from the figure-8 column under similar experimental conditions previously reported. [ABSTRACT FROM PUBLISHER]

Details

Language :
English
ISSN :
10826076
Volume :
38
Issue :
1
Database :
Complementary Index
Journal :
Journal of Liquid Chromatography & Related Technologies
Publication Type :
Academic Journal
Accession number :
98645398
Full Text :
https://doi.org/10.1080/10826076.2014.883533