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A label-free electrochemical biosensor for microRNA detection based on apoferritin-encapsulated Cu nanoparticles.

Authors :
Wang, Mo
Yin, Huanshun
Fu, Zhengliang
Guo, Yunlong
Wang, Xinxu
Zhou, Yunlei
Ai, Shiyun
Source :
Journal of Solid State Electrochemistry; Oct2014, Vol. 18 Issue 10, p2829-2835, 7p
Publication Year :
2014

Abstract

MicroRNAs (miRNAs), a class of small endogenous nonprotein-coding RNAs, regulate a wide range of biological processes, and their abnormal expressions are related to the growth and development of plants. Thus, a simple, rapid, and highly sensitive assay for miRNA detection is of great significance. In this work, a label-free and ultrasensitive assay for miRNA detection using protein cage nanoparticles has been developed. Apoferritin-encapsulated Cu nanoparticles (Cu-apoferritin) could be immobilized on the electrode through special reaction between amino and carboxyl. Hybridization event between the probe DNA and the target miRNA-159a is confirmed by electrochemical oxidation signal after Cu released into the detection buffer by adjusting the pH. This assay is highly selective and sensitive with a low detection limit of 3.5 fM. Moreover, the developed method can even discriminate single-base mismatched strand between the complementary targets. The effect of abscisic acid on the expression level of miRNA-159a in Arabidopsis thaliana seeds was also investigated. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14328488
Volume :
18
Issue :
10
Database :
Complementary Index
Journal :
Journal of Solid State Electrochemistry
Publication Type :
Academic Journal
Accession number :
98405447
Full Text :
https://doi.org/10.1007/s10008-014-2531-y