Mineral trioxide aggregate enhances the odonto/osteogenic capacity of stem cells from inflammatory dental pulps via NF-κB pathway.

Objective This study was designed to investigate the effects of mineral trioxide aggregate (MTA) on the osteo/odontogenic differentiation of inflammatory dental pulp stem cells (i DPSCs). Materials and Methods inflammatory DPSCs were isolated from the inflammatory pulps of rat incisors and cocultured with MTA-conditioned medium. MTT assay and flow cytometry were performed to evaluate the proliferation of i DPSCs. Alkaline phosphatase ( ALP) activity, alizarin red staining, real-time RT- PCR, and Western blot assay were used to investigate the differentiation capacity as well as the involvement of NF-κB pathway in i DPSCs. Results Mineral trioxide aggregate-treated i DPSCs demonstrated the higher ALP activity and formed more mineralized nodules than the untreated group. The odonto/osteoblastic markers ( Alp, Runx2/RUNX2, Osx/OSX, Ocn/OCN, and Dspp/DSP, respectively) in MTA-treated i DPSCs were significantly upregulated as compared with untreated i DPSCs. Mechanistically, cytoplastic phos-P65 and nuclear P65 in MTA-treated i DPSCs were significantly increased in a time-dependent manner. Moreover, the inhibition of NF-κB pathway suppressed the MTA-induced odonto/osteoblastic differentiation of i DPSCs, as indicated by decreased ALP levels, weakened mineralization capacity and downregulated levels of odonto/osteoblastic genes ( Osx, Ocn, and Dspp). Conclusions Mineral trioxide aggregate enhances the odonto/osteogenic capacity of DPSCs from inflammatory sites via activating the NF-κB pathway. [ABSTRACT FROM AUTHOR]