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Establishment of an Enzyme-Linked Immunosorbent Assay and Application on Determination of Ginsenoside Re in Human Saliva.
- Source :
- Planta Medica; 2014, Vol. 80 Issue 13, p1143-1150, 8p
- Publication Year :
- 2014
-
Abstract
- This work describes an immunochemical approach for the quality control of Panax ginseng and a pharmacological study of ginsenoside Re, a major bioactive constituent in P. ginseng, using an enzyme-linked immunosorbent assay. A hybridoma secreting monoclonal antibody against ginsenoside Re was produced by fusing splenocytes immunized with a ginsenoside Re-bovine serum albumin conjugate with the hypoxanthine-aminopterinthymidine-sensitive mouse myeloma SP2/0 cell line. The method, at an effective measuring range of 7.8-500 ng ⋅ mL<superscript>-1</superscript> of ginsenoside Re, successfully detected ginsenoside Re in Chinese traditional herb prescriptions. The results demonstrate that we generated a novel and reliable assay system for measuring ginsenoside Re in Chinese medicines more efficiently. Futhermore, we determined the ginsenoside Re concentrations in the saliva of six healthy adults after the oral administration of a ginseng capsule to study the pharmacokinetics of ginsenoside Re in human saliva. [ABSTRACT FROM AUTHOR]
- Subjects :
- QUALITY assurance
ALTERNATIVE medicine
ANIMAL experimentation
BIOLOGICAL assay
BIOLOGICAL models
BIOPHYSICS
ANALYTICAL chemistry
PHYSICAL & theoretical chemistry
ENZYME-linked immunosorbent assay
GINSENG
RESEARCH methodology
MICE
ORAL drug administration
RESEARCH funding
PLANT extracts
DESCRIPTIVE statistics
IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 00320943
- Volume :
- 80
- Issue :
- 13
- Database :
- Complementary Index
- Journal :
- Planta Medica
- Publication Type :
- Periodical
- Accession number :
- 98042775
- Full Text :
- https://doi.org/10.1055/s-0034-1382959