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Cloning and characterization of BnKNAT2 from Brassica napus.

Authors :
TAO Zhang-sheng
LIU Qiong
WANG Xin-fa
LIU Gui-hua
HUANG Yi
Source :
Chinese Journal of Oil Crop Sciences; 2014, Vol. 36 Issue 3, p300-307, 8p
Publication Year :
2014

Abstract

KNOTTED - like (KNOX) family gene KNAT plays pivotal roles in shoot apical meristem (SAM) development. BnKNAT gene from Brassica napus L. cv Zhongshuang 11 was cloned. The full - length ORF (984bp) included 5 exons encoding 327 amino acids, and 4 specific domains, namely KNOX1, KNOX2, ELK and Homeobox KN. 4 copies of BnKNAT2 were found in B. napus, and were expressed in primordia of main inflorescence, and 7 - day - old pods and stems after pollination. Overexpression construct pD1301S - BnKNAT2 driven by CaMV 35S promoter were conducted and transformed into Arabidopsis wild - type Col - 0 . A total of 21 positive transgenic lines (T2) were obtained, which showed morphologic variations of curled leaves, crouched leaves and corrugated leaf margin. In addition, their flowering date was delayed. [ABSTRACT FROM AUTHOR]

Details

Language :
Chinese
ISSN :
10079084
Volume :
36
Issue :
3
Database :
Complementary Index
Journal :
Chinese Journal of Oil Crop Sciences
Publication Type :
Academic Journal
Accession number :
97159671
Full Text :
https://doi.org/10.7505/j.issn.1007-9084.2014.03.002