Learning from agriculture: understanding low-dose antimicrobials as drivers of resistome expansion.

Antimicrobial resistance is a growing public health challenge worldwide, with agricultural use of antimicrobials being one major contributor to the emergence and dissemination of antimicrobial resistance. Globally, most antimicrobials are used in industrial food animal production, a major context for microbiomes encountering low-?-doses or subtherapeutic-?-levels of antimicrobial agents from all mechanistic classes. This modern practice exerts broad eco-?-evolutionary effects on the gut microbiome of food animals, which is subsequently transferred to animal waste. This waste contains complex constituents that are challenging to treat, including antimicrobial resistance determinants and low-?-dose antimicrobials. Unconfined storage or land deposition of a large volume of animal waste causes its wide contact with the environment and drives the expansion of the environmental resistome through mobilome facilitated horizontal genet transfer. The expanded environmental resistome, which encompasses both natural constituents and anthropogenic inputs, can persist under multiple stressors from agriculture and may re-?-enter humans, thus posing a public health risk to humans. For these reasons, this review focuses on agricultural antimicrobial use as a laboratory for understanding low-?-dose antimicrobials as drivers of resistome expansion, briefly summarizes current knowledge on this topic, highlights the importance of research specifically on environmental microbial ecosystems considering antimicrobial resistance as environmental pollution, and calls attention to the needs for longitudinal studies at the systems level. [ABSTRACT FROM AUTHOR]