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Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway.
- Source :
- BMC Complementary & Alternative Medicine; 2014, Vol. 14 Issue 1, p1-18, 18p, 1 Chart, 4 Graphs
- Publication Year :
- 2014
-
Abstract
- Background Angelica sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. Methods An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. Result The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P < 0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. Conclusion The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANALYSIS of variance
ANIMAL experimentation
BLOOD protein electrophoresis
CELL culture
HYPERTROPHY
MEDICINAL plants
MICE
RESEARCH funding
PLANT roots
STATISTICS
T-test (Statistics)
TRADITIONAL medicine
WESTERN immunoblotting
PLANT extracts
DATA analysis
DONG quai
DATA analysis software
DESCRIPTIVE statistics
IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 14
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary & Alternative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 96042761
- Full Text :
- https://doi.org/10.1186/1472-6882-14-144