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A new transfectant panel cell line-based Mo Ab-independent antigen capture assay system for detection of CD36 antibody.
- Source :
- Vox Sanguinis; May2014, Vol. 106 Issue 4, p368-371, 4p, 1 Diagram, 1 Chart
- Publication Year :
- 2014
-
Abstract
- Background and Objectives CD36 antibody ( Ab) causes several disorders: neonatal alloimmune thrombocytopenia, platelet transfusion refractoriness and non-haemolytic transfusion reactions. However, there is no gold-standard test for CD36 Ab. Materials and Methods We developed a transfectant panel cell line-based Mo Ab-independent antigen capture assay system for detection of CD36 Ab and compared it with the monoclonal antibody-specific immobilization of platelet antigens ( MAIPA) system in terms of sensitivity and specificity. Results Our new system was characterized by (1) gene-transfected cell lines, but not panel platelets; (2) not being hampered by HLA Abs; and (3) no need to use CD36 Mo Abs to ensure the antigen specificity of this detection system. In addition, it showed a much better receiver operating characteristic curve than the MAIPA system. Conclusions The present results indicate that our new system permits highly sensitive and specific detection of CD36 Ab. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00429007
- Volume :
- 106
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Vox Sanguinis
- Publication Type :
- Academic Journal
- Accession number :
- 95661962
- Full Text :
- https://doi.org/10.1111/vox.12118