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Ectopic expression of transcription factor AP-2δ in developing retina: effect on PSA-NCAM and axon routing.
- Source :
- Journal of Neurochemistry; Apr2014, Vol. 129 Issue 1, p72-84, 13p
- Publication Year :
- 2014
-
Abstract
- Retinal ganglion cells transmit the visual signal from the retina to the brain. We have previously shown that the activator protein 2 (AP-2)δ (TFAP2D) transcription factor is expressed in one third of ganglion cells in developing retina suggesting a specialized role for these AP-2δ-expressing cells. Here, we address the role of AP-2δ in retina by in ovo electroporation of RCAS/AP-2δ retroviral constructs into the eyes of chick embryos at day 2 of gestation. Ectopic expression of AP-2δ does not affect lineage differentiation in the developing retina. However, immunostaining of retinal tissue with markers associated with axonal growth such as growth-associated protein 43 and polysialic acid-neural cell adhesion molecule (PSA-NCAM) demonstrates axonal misrouting and abnormal axonal bundling. Treatment of AP-2δ-misexpressing retinal cell cultures with endoneuraminidase, an enzyme that removes PSA from NCAM, decreases AP-2δ-induced axonal bundling. Our data suggest a role for AP-2δ in polysialylation of NCAM, with ectopic expression of AP-2δ resulting in premature bundling of emerging axons and misrouting of axons. We propose that expression of AP-2δ in a subset of ganglion cells contributes to the fine-tuning of axonal growth in the developing retina. [ABSTRACT FROM AUTHOR]
- Subjects :
- ECTOPIC tissue
AXONS
VISUAL pigments
TRANSCRIPTION factors
CYTOLOGICAL techniques
Subjects
Details
- Language :
- English
- ISSN :
- 00223042
- Volume :
- 129
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 95052599
- Full Text :
- https://doi.org/10.1111/jnc.12521