Isolation and Characterization of a New Pseudomonas aeruginosa Strain from Bacterial Stalk Rotted Maize.

To identify the pathogen of maize bacterial stalk rot disease occurred in Zhengzhou, He'nan Province, the strain YM02 was isolated from maize stalk rot samples and then identified and characterized by morphology observation, pathogenicity test, biochemical and physiological characteristics analyses, and molecular phylogeny genetic relationship analysis. The results showed that the isolated strain (YM02) was a Gram-negative pathogenic bacterium; it could ferment several kinds of sugar or alcohol to produce acid, and transfer arabinogalactan into alkali. In addition, the 16S rDNA sequence of YM02 strain had a similarity of above 99% with those of internal and external isolated Pseudomonas aeruginosa strains. Thus, this strain was named as Pseudomonas aeruginosa YM02, which had the closest genetic relationship with Pseudomonas aeruginosa strains ANSC,S164S.,S167S(2) and strains CS-2. The isolation and characterization of this pathogen had provided basic data for the prevention and treatment of maize bacterial stalk rot disease. [ABSTRACT FROM AUTHOR]