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2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination.
- Source :
- PLoS ONE; Feb2014, Vol. 9 Issue 2, p1-5, 5p
- Publication Year :
- 2014
-
Abstract
- We reported a new effective approach to carry out two-photon excitation stimulated emission depletion (2PE-STED) microscopy using a single Ti:sapphire laser system. With an acoustic-optic Bragg cell, the modulated-CW 2PE STED microscope had the benefits of both CW and pulse approaches: lower input power, simple optical scheme and no complicated synchronization. Additionally, it also took advantages of fluorescence yield increasing. The sub-diffraction-limit resolution was demonstrated using ATTO 425-tagged clathrin-coated vesicles. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 94730181
- Full Text :
- https://doi.org/10.1371/journal.pone.0088464