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α-Parvin, a pseudopodial constituent, promotes cell motility and is associated with lymph node metastasis of lobular breast carcinoma.
α-Parvin, a pseudopodial constituent, promotes cell motility and is associated with lymph node metastasis of lobular breast carcinoma.
- Source :
- Breast Cancer Research & Treatment; Feb2014, Vol. 144 Issue 1, p59-69, 11p
- Publication Year :
- 2014
-
Abstract
- Invasive lobular carcinoma (ILC) is more frequently lymph node positive than is invasive ductal carcinoma (IDC), and ILC cell infiltration shows distinctive histological characteristics, suggesting the action of ILC-specific invasion molecules. To identify such a molecule, we used a proteomic approach in the pseudopodia of MDA-MB-231 breast cancer cells. A pseudopodial constituent was identified using excimer laser ablation, two-dimensional difference gel electrophoresis, mass spectroscopy, and immunocytofluorescence. MDA-MB-231 cells were modified to express various levels of this constituent by transient transfection and were examined for pseudopodia formation and migratory abilities using wound healing and two-chamber assays. Immunohistochemical positivity of human breast cancer cells (56 ILCs and 21 IDCs) was compared with clinicopathological variables. An actin-binding adaptor protein, α-parvin, was found to localize to pseudopodia and to form focal adhesions in cells not induced to extend pseudopodia. Pseudopodial length and density and migratory abilities correlated with α-parvin expression. Twenty-one (37.5 %) ILCs stained positive for α-parvin, whereas the results were negative for all 21 IDCs ( P < 0.001). α-Parvin positivity in ILC was significantly associated with lymphatic invasion ( P = 0.038) and lymph node metastasis ( P = 0.003) in univariate analyses and to lymph node metastasis ( P = 0.020) in multivariate analyses. α-Parvin, a pseudopodial constituent, was found to promote migration of breast cancer cells and to be expressed exclusively by ILC, suggesting that α-parvin is an ILC-specific invasion molecule that may have clinical utility as a biomarker for aggressive subsets of ILC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01676806
- Volume :
- 144
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Breast Cancer Research & Treatment
- Publication Type :
- Academic Journal
- Accession number :
- 94420371
- Full Text :
- https://doi.org/10.1007/s10549-014-2859-0