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Expression, purification, and bioactivity of (GLP-1)-HSA analogs in Pichia pastoris GS115.
- Source :
- Biotechnology & Bioprocess Engineering; Nov2013, Vol. 18 Issue 6, p1076-1082, 7p
- Publication Year :
- 2013
-
Abstract
- We developed (GLP-1)-HSA (GGH) analogs that are resistant to degradation and also show high serum glucose-reducing activity in vivo. Five analogs with N-terminal extensions were designed based on the protein GGH. Next, we constructed recombinant plasmids capable of expressing the five analogs in methylotrophic yeast Pichia pastoris GS115. Expression reached 150 mg/L in a small-scale incubation. Fusion proteins were successfully purified from the supernatant using ultrafiltration concentration, affinity absorption chromatography, hydrophobic chromatography, ion exchange chromatography and gel filtration. A single band was observed on SDS-PAGE and the purity was 97%. Activity test results suggested that both A-GGH and G-GGH showed better activity in vitro and that their cAMP levels were significantly increased by 10-fold compared to GGH without N-terminal extension. Additionally, A-GGH efficiently enhanced the glucoselowering effect, which was maintained after the administration for 24 h. A-GGH is a potential drug for treating type 2 diabetes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 12268372
- Volume :
- 18
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Biotechnology & Bioprocess Engineering
- Publication Type :
- Academic Journal
- Accession number :
- 93448022
- Full Text :
- https://doi.org/10.1007/s12257-013-0356-7