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Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia.
- Source :
- Chinese Journal of Integrative Medicine; Dec2013, Vol. 19 Issue 12, p905-912, 8p
- Publication Year :
- 2013
-
Abstract
- Objective: To explore differences in bone marrow angiogenesis seen in aplastic anemia (AA) patients presenting with differential Chinese medicine (CM) syndrome, and to correlate these differences with clinical pathology. Methods: Thirty-five patients were enrolled, including 18 with “yang deficiency syndrome” and 17 with “yin deficiency syndrome.” Bone marrow biopsies and serum were collected. Microvessel density (MVD) and positive expression of vascular endothelial-derived growth factor (VEGF) were detected by immunohistochemisty. Hypoxia inducible factor −1α (HIF-1α), and VEGF expression were assayed by enzyme-linked immunoabsorbent assay (ELISA), serum lactate dehydrogenase (LDH) was tested by enzyme method and liquid chip technology was used to detected the expression of interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN)-γ and tumor necrosis factor (TNF)-α. Results: Counts for leukocytes, absolute neutrophils and platelets in “yin deficiency syndrome” were lower than those found in “yang deficiency syndrome” ( P<0.05). MVD and VEGF expression, and the positive rate of CD34 and VEGF in bone marrow were lower in AA, especially in “yin deficiency syndrome” ( P<0.01 or P<0.05). “Yin deficiency syndrome” displayed decreased VEGF and LDH expression, and enhanced expression of HIF-1α as compared to “yang deficiency syndrome” ( P<0.05). Levels of IL-4 and IL-6 were higher in AA ( P<0.01), but IL-10 was decreased ( P<0.05). High TNF-α expression was seen in “yang deficiency syndrome” and IFN-γ expression was decreased in “yin deficiency syndrome” as compared with normals ( P <0.01 and P<0.05, respectively). Conclusion: AA patients have lower MVD than normals, especially in “yin deficiency syndrome.” MVD might differentially correlate to disease severity, and could be dependent on bone marrow or serum VEGF expression and LDH. Additionally, IL-2, IL-10, IL-4 and IFN-γ were negatively associated while IL-6 and TNF-α were positively associated with MVD. [ABSTRACT FROM AUTHOR]
- Subjects :
- BIOPSY
APLASTIC anemia
BLOOD platelets
BONE marrow
CYTOKINES
ENZYME-linked immunosorbent assay
EXPERIMENTAL design
IMMUNOHISTOCHEMISTRY
INTERFERONS
INTERLEUKINS
LACTATE dehydrogenase
CHINESE medicine
NEUTROPHILS
REGRESSION analysis
RESEARCH funding
STAINS & staining (Microscopy)
TUMOR necrosis factors
SYNDROMES
VASCULAR endothelial growth factors
DATA analysis software
LEUKOCYTE count
PATHOLOGIC neovascularization
PROGNOSIS
DIAGNOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 16720415
- Volume :
- 19
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Chinese Journal of Integrative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 92704849
- Full Text :
- https://doi.org/10.1007/s11655-013-1652-y