Human-milk Intake measured by administration of deuterium oxide to the mother: a comparison with the test-weighing technique.

A comparison was made between the dose-to-the-mother deuterium-dilution method and the conventional test-weighing technique for determining human-milk intake in five exclusively breast-fed infants and in four breast-fed infants who received supplemental foods. After administration of ²H to the mothers human milk and infant urine were sampled over 14 d and analyzed for ²H:¹H ratios by gas-isotope-ratio mass spectrometry. Infant total body water was determined by l80 dilution. The test-weighing procedure was conducted for 5 d consecutively. The intake of human milk (mean ± SD) estimated by ²H dilution was 648 ± 63 g/d and estimated by test-weighing was 636 ± 84 g/d. The mean difference between the two methods was not significantly different from 0. The ²H-dilution and test-weighing techniques provide similar estimates of human-milk intake. [ABSTRACT FROM AUTHOR]