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Identification of neglected cestode Taenia multiceps microRNAs by illumina sequencing and bioinformatic analysis.

Authors :
Xuhang Wu
Yan Fu
Deying Yang
Yue Xie
Runhui Zhang
Wanpeng Zheng
Huaming Nie
Ning Yan
Ning Wang
Jiahai Wang
Xiaobin Gu
Shuxian Wang
Xuerong Peng
Guangyou Yang
Source :
BMC Veterinary Research; 10/1/2013, Vol. 9 Issue 1, p1-13, 13p
Publication Year :
2013

Abstract

Background: Worldwide, but especially in developing countries, coenurosis of sheep and other livestock is caused by Taenia multiceps larvae, and zoonotic infections occur in humans. Infections frequently lead to host death, resulting in huge socioeconomic losses. MicroRNAs (miRNAs) have important roles in the post-transcriptional regulation of a large number of animal genes by imperfectly binding target mRNAs. To date, there have been no reports of miRNAs in T. multiceps. Results: In this study, we obtained 12.8 million high quality raw reads from adult T. multiceps small RNA library using Illumina sequencing technology. A total of 796 conserved miRNA families (containing 1,006 miRNAs) from 170,888 unique miRNAs were characterized using miRBase (Release 17.0). Here, we selected three conserved miRNA/miRNA" (antisense strand) duplexes at random and amplified their corresponding precursors using a PCR-based method. Furthermore, 20 candidate novel miRNA precursors were verified by genomic PCR. Among these, six corresponding T. multiceps miRNAs are considered specific for Taeniidae because no homologs were found in other species annotated in miRBase. In addition, 181,077 target sites within T. multiceps transcriptome were predicted for 20 candidate newly miRNAs. Conclusions: Our large-scale investigation of miRNAs in adult T. multiceps provides a substantial platform for improving our understanding of the molecular regulation of T. multiceps and other cestodes development. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17466148
Volume :
9
Issue :
1
Database :
Complementary Index
Journal :
BMC Veterinary Research
Publication Type :
Academic Journal
Accession number :
91269824
Full Text :
https://doi.org/10.1186/1746-6148-9-162