Back to Search
Start Over
Enhanced Expression of the Inhibitory Protein Gi2α and Decreased Activity of Adenylyl Cyclase in Lymphocytes of Abstinent Alcoholics.
- Source :
- Alcoholism: Clinical & Experimental Research; 1993, Vol. 17 Issue 2, p315-320, 6p
- Publication Year :
- 1993
-
Abstract
- Ethanol exposure alters signal transduction through the adenylyl cyclase (AC) system. To elucidate the basis for this effect, we investigated the AC system in peripheral lymphocytes from abstinent alcoholic men ( n= 22), actively drinking alcoholic men ( n= 41), and nonalcoholic control men ( n= 16). Immunoblot analysis of lymphocyte membranes from abstinent alcoholics demonstrated a 3.0-fold increase in the level of G<subscript>i2</subscript>α protein ( p < 0.05) compared with controls. However, levels of G<subscript>i2</subscript>α protein were similar in both groups. Abstinent alcoholics had a 2.9-fold increase in G<subscript>i2</subscript>α mRNA ( p < 0.001) and a 2.7-fold increase in G<subscript>2</subscript>α mRNA ( p < 0.03) compared with lymphocytes from control subjects. Actively drinking alcoholics, in contrast, had unaltered G<subscript>2</subscript>α protein, G<subscript>i2</subscript>α protein, and G<subscript>i2</subscript>α mRNA levels compared with control subjects, but did have a 1.8-fold increase ( p < 0.01) in G<subscript>i2</subscript>α mRNA. Consistent with enhanced G<subscript>i2</subscript>α expression, lymphocyte membranes from abstinent alcoholics had decreased basal, prostaglandin E<subscript>1-</subscript>, guanosine 5′-0-(3-thiotriphosphate)-γS-, and forskolin-stimulated AC activity compared with both controls and actively drinking alcoholics ( p < 0.05). We conclude that lymphocyte AC is reduced during abstinence from alcohol and enhanced expression of the inhibitory G-protein, G<subscript>i2</subscript>α, may account for this change. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- Volume :
- 17
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Alcoholism: Clinical & Experimental Research
- Publication Type :
- Academic Journal
- Accession number :
- 91181716
- Full Text :
- https://doi.org/10.1111/j.1530-0277.1993.tb00769.x