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Short communication: point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Colombia.
- Source :
- Tropical Medicine & International Health; Feb2003, Vol. 8 Issue 2, p129-132, 4p
- Publication Year :
- 2003
-
Abstract
- Point mutations in the dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) genes of Plasmodium falciparum can lead to an increasing resistance of P. falciparum to pyrimethamine/sulphadoxine. We examined the prevalence of these mutations in 36 samples from Colombia. Analysed by polymerase chain reaction (PCR) for infection with P. falciparum, 25 (69%) tested positive. These positive isolates were tested further for point mutations in the genes of DHFR (codons 16, 51, 59, 108 and 164) and DHPS (codons 436, 437, 540, 581 and 613) by nested PCR and following mutation-specific restriction enzyme digestion. Gene mutations occurred in both the DHFR and DHPS gene of the Colombian isolates, suggesting that resistance to antifolate drugs exists or may develop soon in Colombia. [ABSTRACT FROM AUTHOR]
- Subjects :
- PLASMODIUM falciparum
GENETICS
Subjects
Details
- Language :
- English
- ISSN :
- 13602276
- Volume :
- 8
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Tropical Medicine & International Health
- Publication Type :
- Academic Journal
- Accession number :
- 9079319
- Full Text :
- https://doi.org/10.1046/j.1365-3156.2003.01005.x